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The opening title card to the film reads "Republic Pictures presents The Three Mesquiteers in Riders of the Rio Grande," followed by pictures of Bob Steele, Tom Tyler and Jimmie Dodd with their names and character names superimposed. Modern sources include John James, Henry Hall and Yakima Canutt in the cast. This was the last entry in The Three Mesquiteers series, which began in 1936. For more information on the series, consult the Series Index and the entry for The Three Mesquiteers (see AFI Catalog of Feature Films, 1931-40; F3.4617).