Batman and Robin battle Catwoman, the Penguin, the Joker and the Riddler, who seek to defeat the Dynamic Duo once and for all, and take over the world.

Batman, the caped crusader, and Robin, the boy wonder, learn that Commodore Schmidlapp, a millionaire distiller, is in danger aboard his yacht and rush to his aid by batcopter, but the yacht disappears beneath Batman as he is descending the batladder. The dynamic duo learns that The Catwoman, The Joker, The Penguin, and The Riddler have joined forces to eliminate Batman and Robin and eventually control the world. (The vanishing yacht was a decoy to lure Batman and Robin from the place where the villains were hijacking the yacht of Commander Redhead and holding him prisoner because they want his invention, a dehydrater that turns humans into dust that can be returned to human form with the addition of water.) After many encounters the villains are still loose, and The Penguin goes to Security Council headquarters. There he turns nine diplomats into dust, which he places in nine test tubes. Batman and Robin, however, outwit and capture the quartet of criminals and rescue Commander Redhead. As they are about to reconstitute the nine diplomats, the commander sneezes on the test tubes, scattering the dust. Batman does his best to sort the dust into nine homogeneous piles, but when the dust is restored to human form, the Russian speaks Greek, the Greek speaks English, and so on. But Batman is satisfied that the world is safe from the four fiends for the time being.